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[KD]Flotillin 1 Rabbit mAb

Western blot analysis of Flotillin 1 in HeLa(WT) and Flotillin 1-Knockdown HeLa(KD) using Flotillin 1 antibody.
Western blot analysis of Flotillin 1 in K562, Hela lysates using Flotillin 1 antibody.
Immunocytochemistry analysis of Flotillin 1(green) in Hela using Flotillin 1 antibody,and DAPI(blue)

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, IP
Primary Accession	O75955
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	47355 Da

Additional Information
Gene ID	10211
Other Names	FLOT1
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200 FC~~1:20-1:50 IP~~1:10-1:100
Format	Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FLOT1
Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cellular Location	Cell membrane; Peripheral membrane protein. Endosome Membrane, caveola {ECO:0000250\|UniProtKB:O08917}; Peripheral membrane protein {ECO:0000250\|UniProtKB:O08917}. Melanosome. Membrane raft. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Membrane-associated protein of caveola (By similarity) {ECO:0000250\|UniProtKB:O08917, ECO:0000269\|PubMed:17081065}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants.

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¥ 1,250.00

Cat# AP75443

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[KD]Flotillin 1 Rabbit mAb

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